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  Indian J Med Microbiol
 

Figure 3 The initial culture of the isolated stem cells contain a crowded cell population with a majority of small spherical cells (a, b). A gradual decrease in the number of rounded cells (c). The appearance of fibroblast-like mesenchymal stem cells (d). Osteogenic differentiation of mesenchymal stem cells into osteoblasts and acquiring of Alizarin red S stain (e and f) (a and b, ×20; C–F, ×40).

Figure 3 The initial culture of the isolated stem cells contain a crowded cell population with a majority of small spherical cells (a, b). A gradual decrease in the number of rounded cells (c). The appearance of fibroblast-like mesenchymal stem cells (d). Osteogenic differentiation of mesenchymal stem cells into osteoblasts and acquiring of Alizarin red S stain (e and f) (a and b, ×20; C–F, ×40).